The expression of resistin-like molecules-a in atherosclerotic plaque

( views:, downloads: )
Author:
ZHANG Hong-ming(Department of Cardiology, The General Hospital of Jinan Military Region, Jinan 250031, China)
LI Xiiao-yan(Department of Cardiology, The General Hospital of Jinan Military Region, Jinan 250031, China)
HE Zuo-yun()
TAN Hong(Department of Cardiology, The General Hospital of Jinan Military Region, Jinan 250031, China)
LIU Ke-wei(Department of Cardiology, The General Hospital of Jinan Military Region, Jinan 250031, China)
HUANG Zhi-gang(Department of Cardiology, The General Hospital of Jinan Military Region, Jinan 250031, China)
Journal Title:
CHINESE JOURNAL OF EMERGENCY MEDICINE
Issue:
Volume 18, Issue 07, 2009
DOI:
10.3760/cma.j.issn.1671-0282.2009.07.012
Key Word:
Artherosclerosis;RELMα; Vascular smooth muscles cells;Proliferation

Abstract: Objective To explore the expression of resistin-like molecules-a (RELMa) in atherosclerotic plaque of ApoE-/- mouse, and to study the effects of RELMa on the proliferation and migration of vascular smooth musclee cells (VSMCs) . Method Nine ApoE-/- mice and nine C57BL/6J mice were fed with high fat diet. All mice were sacrificed 24 weeks after force feeding. Vessels were dissected from to abdominal aorta. Sections of aortic tissue were stained with HE dyeing and RELMa in aortic tissue was assayed by immunohistochemistry. The expression of RELMa mRNA in vessels was detected by RT-PCR. The effects of different concentration RELMa in different concentrations on the proliferation and migration of VSMCs were detected. Data was expressed as mean ± standard deviation. ANOVA were used for comparison in SPSS 11.0, and changes were considered as statistically significant if P value was less than 0.05. Results Atherosclerosis plaque formed in aortic root of ApoE-/- mice after they were fed with high fat diet for 24 weeks. RELMa protein and RELMa mRNA were found by immunohistochemistry and RT-PCR in atherosclerotic plaque of ApoE-/- mouse. RELMa protein didn't be found in vessels of control mouse. RELMa promoted the proliferation of VSMCs (RELMa groups: 2811. 21 ± 216. 89,4056. 87 ±220.65,5061.45 ± 335.86, vs. control 1609.58 ± 203.53, P < 0.01). RELMa promoted the migration of VSMCs (RELMa groups: 130.54±12.98,158.39±11.58,203.50± 17.37 vs. control:70.54± 11.92, P<0.01).Conclusions RELMa expresses in atherosclerotic plaque of ApoE-/- mouse. RELMa enhances the proliferation and migration of VSMCs of aorta.

  • [1]Rajala MW,Lin Y,Ranalletta M,et al.Cell type-specific expression and coregulation of murine resistin and resistin-like molecule -alpha in adipose tissue[J].Mol Endocrinol,2002,16(8):1920-1930.
  • [2]Shojima N,Ogihara T,Inukai K,et al.Serum concentrations of resistinlike molecules beta and gamma are elevated in high-fat-fed and obese db/db mice,with increased production in the intestinal tract and bone marrow[J].Diabetologia,2005,48(5):984-992.
  • [3]Kushiyama A,Shojima N,Ogihara T,et al.Resistin like molecule beta activates MAPKs,suppresses insulin signaling in hepatocytes and induces diabetes,hyperlipidemia and fatty liver in transgenic mice on a high-fat diet[J].J Biol Chem,2005,280(51):42016-42025.
  • [4]Jung HS,Park KH,Cho YM,et al.Resistin is secreted from macrophages in atheromas and promotes atherosclerosis[J].Cardiovasc Res,2006,69(1):76-85.
  • [5]Teng X,U D,Champion HC,et al.FIZZI/RELMalpha,a novel hypoxia-induced mitogenic factor in lung with vasoconstrictive and angiogenic properties[J].Circ Res,2003,92(10):1065-1067.
  • [6]Wilson MS,Mentink-Kane MM,Pesce JT,et al.Immunopathology of schistosomiasis[J].Jmmunol Cell Biol,2007,85(2):148-154.
  • [7]Trujillo G,O' Connor EC,Kunkel SL,et al.A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis[J].Am J Pathol,2008,172(5):1209-1221.
  • [8]Donnelly S,O'Neill SM,Sekiya M,et al.Thioredoxin peroxidase secreted by Fasciola hepatica induces the alternative activation of macrophages[J].Infect Immun,2005,73(1):166-173.
  • [9]Mora AL,Torres-González E,Rojas M,et al.Activation of alveolar macrophages via the alternative pathway in herpesvirus-induced lung fibrosis[J].Am J Respir Cell Mol Biol,2006,35(4):466-473.
  • [10]冯宗忱.动脉粥样硬化-血管壁的慢性炎症[J].中华心血管病杂志,2005,33(5):393-394.
  • [11]Joshi AD,Raymond T,Coelho AL,et al.A systemic granulomatous response to Schistosoma mansoni eggs alters responsiveness of bonemarrow-derived macrophages to Toll-like receptor agonists[J].J Leukoc Biol,2008,83(2):314-324.
  • [12]Persaud R,Wang A,Reardon C,et al.Characterization of the immuno-regulatory response to the tapeworm Hymenolepis diminuta in the non-permissive mouse host[J].Int J Parasitol,2007,37(3):393-403.
  • [13]李欣,廖新学,廖晓星,等.银杏叶提取物对动脉粥样硬化的防治作用[J].中华急诊医学杂志,2004,13(9):609-611.
  • [14]Tong Q,Zheng L,Lin L,et al.Hypoxia-induced mitogenic factor promotes vascular adhesion molecule-1 expression via the PI-3K/Akt-NF-{ kappa }B signaling pathway[J].Am J Respir Cell Mol Biol,2006,35(4):444-456.
  • [15]Sun Y,Wang J,Li H,Found in inflammatory zone 1 induces angiogenesis in murine models of asthma[J].Lung,2008,186(6):375-380.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn