The effect of activating retinoid X receptor inhibiting hydrogen peroxide-induced apoptosis in cultured rat neonatal cardiomyocytes

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Author:
SHAN Pei-ren(Cardiovascular Department,Renji Hospital,Shanghai Jiaotong University Medical College,Shanghai 200127,China)
YUAN An-cai(Cardiovascular Department,Renji Hospital,Shanghai Jiaotong University Medical College,Shanghai 200127,China)
WANG Li-min()
SHEN Ling-hong(Cardiovascular Department,Renji Hospital,Shanghai Jiaotong University Medical College,Shanghai 200127,China)
CHAI Da-jun(Cardiovascular Department,Renji Hospital,Shanghai Jiaotong University Medical College,Shanghai 200127,China)
ZHOU Lei(Cardiovascular Department,Renji Hospital,Shanghai Jiaotong University Medical College,Shanghai 200127,China)
PU Jun(Cardiovascular Department,Renji Hospital,Shanghai Jiaotong University Medical College,Shanghai 200127,China)
HE Ben(Cardiovascular Department,Renji Hospital,Shanghai Jiaotong University Medical College,Shanghai 200127,China)
Journal Title:
CHINESE JOURNAL OF EMERGENCY MEDICINE
Issue:
Volume 17, Issue 05, 2008
DOI:
Key Word:
Retinoid X receptor;Cardiomyoeytes;Apoptosis;Oxidative stress

Abstract: Objective To investigate the effect of 9-cis retinoid acid(c-RA),a retinoid X receptor(RXR)agonist,on hydrogen peroxide(H2O2)induced apoptosis in cultured rat neonatal cardiomyoeytes,and to explore the mechanism.Method Cultured cardiomyocpes were randomly divided into three groups:normal group treated with vehicle(N group),H2O2 group treated with 100 μmol/L H2O2(H group),and c-RA group pretreated with 100nmol/L c-RA(H+R group).Cell viability was detected by MTT.Morphological changes of apoptotic cardiomyocytes were observed by Hoechst 33258 staining under fluorescence microscope.The apoptotic rate was determined by flow cytometry.Mitochondrial membrane potential(△(ψ)m)was measured by JC-1 dye.Cellular reactive oxygen species(ROS)production was detected by CM-H2DCFDA fluorescent probe.All measurement data wIe expressed as(x±s),and statistically analyzed using one-way ANOVA analysis and Dunnett test.Differences were considered significant when P was<0.05.Results Treatment with c-RA significantly enhanced cell viability,reduced apoptosis ratio,stabled mitoehondrial membrane potential and reduced level of cellular reactive oxygen species.Conclusions RXR agonist c-RA inhibits H2O2-induced myocyte apoptosis in cultured rat neonatal cardiomyocytes,which may be related to alleviate oxidative stress injury.

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