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Dynamic changes of serum matrix metalloproteinase-9(MMP-9)and its significance in rat model of alcoholic liver disease with Vibrio vulnificus sepsis
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 3
- DOI:
- 10.3760/j.issn:1671-0282.2008.03.005
- Key Word:
- 脓毒症;创伤弧菌;病理形态学;毒血症状;基质金属蛋白酶-9
Abstract： 目的 探讨血清MMP-9水平与酒精性肝病大鼠创伤弧菌脓毒症进展的关系.方法 制作酒精性肝病大鼠创伤弧菌脓毒症模型,分脓毒症组及抗菌药物干预组.观察脓毒症组大鼠在染菌后2 h、6 h、12 h、24 h各时间点的脓毒症表现及光镜下肺组织病理改变,并以ELISA法测定脓毒症组及抗菌药物干预组大鼠染菌后各时间点血清MMP-9水平.计量资料采用(x±S)表示.采用SPSS 10.0进行统计,各组血清MMP-9值作t检验,多组间差异采用单囚素方差分析处理.P＜0.05为差异有统计学意义.结果 脓毒症组大鼠染菌后6 h开始出现较明显的毒血症状,并随时间的延长加剧,24 h达高峰,濒临死亡.光镜下肺组织损伤以炎症细胞浸润及肺泡壁水肿充血为主,6 h较轻,12 h损伤加重,到24 h可见大量的炎细胞浸润,淋巴滤泡形成,伴肺泡腔出血.脓毒症组血清MMP-9水平染菌后6 h明显升高(P＜0.01),12 h达高峰,24 h已明显下降(P＜0.01,与12 h组比).抗菌药物干预组大鼠血清MMP-9变化曲线与脓毒症组一敛,但与脓毒症组相应时间点比较,仅6 h时MMP-9水平明显减低(P＜0.01).结论 MMP-9可能不是酒精性创伤弧菌脓毒症后期病情恶化的主要参与因素,血清MMP-9水平不能准确地反应酒精性肝病创伤弧菌脓毒症病情轻重及全身器官损伤的程度.
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