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The combined effects of ligustrazine and hypertonic saline on cerebral resuscitation after cardiac arrest in rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1671-0282.2008.01.004
- Key Word:
- 川芎嗪;高渗盐;心搏骤停;脑复苏;丙二醛
Abstract： 目的 研究川芎嗪联合高渗盐对心搏骤停大鼠复苏后脑组织的保护作用,探讨脑复苏治疗的有效方法.方法 32只SD大鼠被随机分成4组(每组8只):生理盐水组、川芎嗪组、高渗盐水组、川芎嗪联合高渗盐水组.窒息导致心搏骤停模型复制成功后,实验各组于复苏即刻分别静脉注射生理盐水、川芎嗪、高渗盐水、川芎嗪联合高渗盐水,比较各组大鼠自主循环恢复(ROSC)时间、动脉血及脑匀浆丙二醛(MDA)、脑干湿重比(D/W)、平均动脉压(MAP)、神经功能缺损评分(NDS)及脑海马组织病理改变.结果 与对照组相比,联合用药组、高渗盐组、川芎嗪组大鼠自主循环恢复时间(P＜0.05)明显改善,10 min、30 min和60 min的平均动脉压明显提高,24、48 h较对照组明显改善NDS(P＜0.01),大鼠48 h存活率显著提高,大脑干湿重比值增加(P＜0.05)及脑组织病理损伤减轻;联合用药组较川芎嗪组、高渗盐组上述观测指标有显著改善.与对照组相比,联合用药组及川芎嗪组1 h动脉血MDA明显降低(P＜0.05),48 h脑匀浆MDA降低(P＜0.05),联合用药组较川芎嗪组1小时动脉血、48 h脑匀浆MDA亦明显降低;与对照组相比,高渗盐组1 h动脉血MDA差异无统计学意义(P＞0.05),48 h脑匀浆MDA差异亦无统计学意义(P＞0.05);且高渗盐组较川芎嗪组ROSC时间、ROSC后60 min MAP、24和48 h NDS、大脑干湿重比差异无统计学意义(P＞0.05).结论 静脉注射川芎嗪联合高渗盐能减轻大鼠心跳呼吸骤停复苏后脑组织损伤,改善脑功能;联合应用两药可提高疗效.
- [1]黄子通,符岳.心搏骤停后脑缺血缺氧损害与脑复苏[J].2006,(1).
- [2].窒息法致大鼠心脏骤停模型复苏的影响因素[J].2005,(10).
- [3]吴光勇,毕丹东,刘运生,等.川芎嗪在不同时期对脑缺血再灌注后MMP-9表达的影响[J].2005,(6).
- [4]林海,刘煜,李柱一,等.局灶性脑缺血再灌注后白细胞介素-6、肿瘤坏死因子-α的表达及川芎嗪对其表达的影响[J].2002,(7).
- [5]庄明华,白晔,黄集前,等.共聚焦激光扫描显微镜活体观测川芎嗪和去甲基肾上腺素对生理状态下家兔大脑皮质内微循环的影响[J].2004,(1).
- [6]俞海国,赵燕,汤云珍,等.川芎嗪对新生鼠缺氧缺血性脑损伤c-fos基因表达影响的研究[J].2001,(2).
- [7]Fritz Sterz,Yuval Leonov,Peter Safar,etc.Multifocal cerebral blood flow by Xe-CT and global cerebral metabolism after prolonged cardiac arrest in dogs. Reperfusion with open-chest CPR or cardiopulmonary bypass[J].1992,24(1).
- [8]Claire, Battison,Peter J D, Andrews,Catriona, Graham,etc.Randomized, controlled trial on the effect of a 20% mannitol solution and a 7.5% saline/6% dextran solution on increased intracranial pressure after brain injury.[J].2005,33(1).
- [9]Bhardwaj A,Ulatowski JA.Hypertonic saline solutions in brain injury.[J].2004,10(2).
- [10]Ann Radovsky,Laurence Katz,Uwe Ebmeyer,etc.Ischemic Neurons in Rat Brains After 6, 8, or 10 Minutes of Transient Hypoxic Ischemia[J].1997,25(5).
- [11]L, Katz,U, Ebmeyer,P, Safar,etc.Outcome model of asphyxial cardiac arrest in rats.[J].1995,15(6).
- [12]Ogden AT,Mayer SA,Connolly ES Jr.Hyperosmolar agents in neurosurgical practice: the evolving role of hypertonic saline.[J].2005,57(2).
- [13]王万铁,陈寿权.川芎嗪注射液抗脑缺血-再灌注损伤作用机制的实验研究[J].2001,010 (3).
- [14]Ebmeyer U,Bircher N,Alexander H,etc.Improved cerebral resuscitation from cardiac arrest in dogs with mild hypothermia plus blood flow promotion.[J].1996,27(1).
- [15]T S, Richmond.Cerebral resuscitation after global brain ischemia: linking research to practice.[J].1997,8(2).