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Expression of protein kinase C and extracellular signal regulated protein kinase in severe pancreatitis rats with acute lung injury
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 7
- DOI:
- 10.3760/j.issn:1671-0282.2007.07.006
- Key Word:
- 重症胰腺炎;内毒素;急性肺损伤;蛋白激酶C;细胞外信号调节激酶
Abstract： 目的 探讨重症胰腺炎并急性肺损伤大鼠肺组织蛋白激酶C(PKC)与细胞外信号调节激酶(ERK)的表达及意义.方法 健康成年雄性SD大鼠40只,随机分为对照组(n=8)与胰腺炎组,胰腺炎组再按6、12、24、48 h分(B1、B2、B3、B4)亚组.以大鼠重症胰腺炎所致ALI为模型,采用酶联免疫吸附(ELISA)法检测血清TNF-α、IL-1β及IL-6,Western blot检测肺组织PKC与ERK的蛋白表达,EMSA检测NF-κB活性变化,观察肺脏含水量、肺脏病理变化.结果 内毒素致重症胰腺炎并发急性肺损伤早期即发生肺血管通透性增高,肺脏含水量上升,组织学显示肺脏出现明显的病理损害,血清TNF-α、IL-1β及IL-6增高,肺组织PKC、ERK与NF-κB DNA活性同步增强.结论 PKC与ERK的活化参与了重症胰腺炎时ALI的发病,即ERK/NF-κB信号通路的活化.
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