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Effects of nuclear factor-κB activation in acute lung injury induced by acute severe pancreatitis and the regulatory effects of different drugs
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 4
- DOI:
- 10.3760/j.issn:1671-0282.2007.04.008
- Key Word:
- 急性坏死性胰腺炎;急性肺损伤;肺泡巨噬细胞;核因子-κB;诱生性一氧化氮合酶
Abstract： 目的 探讨核转录因子-kappaB(NF-κB)在急性重症胰腺炎肺损伤发病中的作用及不同药物的影响.方法 经胆胰管逆行注射去氧胆酸钠复制大鼠急性重症胰腺炎肺损伤模型.采用凝胶电泳迁移率改变.(EMSA)法检测肺泡巨噬细胞中NF-κB活性,同时应用逆转录-聚合酶链式反应(RT-PCR)检测肺泡巨噬细胞中诱生性一氧化氮合酶基因(iNOS mRNA)的表达情况,并检测一氧化氮(NO)、肿瘤坏死因子α(TNF-α)、诱生型一氧化氮合酶(iNOS)的含量和肺组织病理学改变.结果 模型组NF-κB活性增强、iNOS mRNA出现高表达,相应的TNF-a、NO、iNOS均明显高于假手术组.地塞米松和善宁可以下调NF-κB活性,减少iNOSmRNA表达,减轻肺损伤程度,而凯复定则无明显作用.结论 急性重症胰腺炎肺损伤时,NF-κB活化,进而启动TNF-α、iNOS、NO大量表达,在发病中起着十分重要的作用.地塞米松和善宁可以通过调节NF-κB活化等多种途径,防止肺组织的进一步损害.
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