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Protective effects of ulinastatin on acute lung injury resulting from sepsis in rat model
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 2
- DOI:
- 10.3760/j.issn:1671-0282.2007.02.005
- Key Word:
- 急性肺损伤;脓毒症;乌司他丁;糖皮质激素
Abstract： 目的 探讨乌司他丁(UTI)对脓毒症性急性肺损伤(ALI)的保护作用.方法 采用盲肠结扎穿孔(CLP)制作SD大鼠脓毒症ALI模型,随机分ALI组、糖皮质激素(GC)治疗(GC)和UTI治疗(UTI)组.成模后3 h、6 h、12 h开腹抽血行动脉血气分析,提取支气管肺泡灌洗液(BALF)检测总蛋白(TP)、总磷脂(TPL)、饱和磷脂酰胆碱(DSPC)含量,测定肺组织湿/干重(W/D)比值和肺组织匀浆中髓过氧化物酶(MPO)、丙二醛(MDA)含量,血浆中肿瘤坏死因子(TNF-α)、白介素-6(IL-6)含量,观察与比较各组肺组织病理改变.结果 UTI组PaCO2、PaO2、HCO3-、BE与GC组差异无统计学意义(P＞0.05),但高于ALI组(P＜0.05);两组W/D均明显低于ALI组(P＜0.01),BALF中TP显著低于ALI组(P＜0.01),TPL和DSPC/TPL高于ALI组(P＜0.05、P＜0.01),但两组间差异无统计学意义(P＞0.05);两组肺组织匀浆中MPO、MDA含量均明显低于ALI组(P＜0.01),UTI组MPO含量还明显高于GC组(P＜0.05);两组血浆TNF-α、IL-6水平均低于ALI组(P＜0.01),UTI组IL-6高于GC组(P＜0.05);两组病理变化均较ALI组轻(P＜0.01).结论 UTI能改善缺氧、过度通气和酸中毒,减轻肺水肿和肺组织病理损伤,具有抗氧化和抑制炎性细胞因子释放作用,其抗炎作用与GC相似.
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