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Intravenous administration of embryonic stem cells home into injured hearts and improve cardiac function post myocardial infarction in rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 12
- DOI:
- 10.3760/j.issn:1671-0282.2006.12.006
- Key Word:
- 胚胎干细胞;心肌梗死;TNF-α;细胞治疗/移植
Abstract： 目的 阐明大鼠急性心肌梗死后静脉注射的胚胎干细胞(ESC)能否定位于损伤心肌和改善心功能,并探讨炎症因子TNF-α的可能作用.方法 ESC体外培养并转染绿荧光蛋白(GFP);左冠前降支结扎建立大鼠心肌梗死后心功能不全模型,手术后每隔天尾静脉注射107ESC共6 d,并以心肌梗死非细胞治疗组和假手术组为对照.6周后血流动力学方法测定心功能,免疫组织化学法检测心脏和其它脏器中GFP阳性区和心肌组织中GFP阳性细胞的心肌特异性蛋白肌钙蛋白Ⅰ(Tn Ⅰ)的表达.另外,应用细胞共培养观察了TNF-α过度表达的心肌细胞对ESC迁移的影响.结果 以心肌梗死后非细胞治疗组为对照,6周后ESC治疗组心功能显著改善;组织学检测发现ESC治疗组在心肌梗死区可见GFP阳性区,并相应于GFP阳性区Tn Ⅰ染色阳性.在其他脏器中,仅在脾脏组织中可见少量的GFP阳性细胞,提示循环ESC定位于梗死损伤心肌并形成心肌组织.体外ESC迁移的研究发现TNF-α过度表达的心肌细胞可显著增加ESC的迁移.结论 经静脉注射的循环ESC可定向迁移至急性心肌梗死后的损伤心肌,并进一步分化为心肌细胞而改善心功能.体外研究提示这可能与急性心肌梗死时局部释放的炎症因子如TNF-α对循环ESC的趋化作用有关.
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