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Effects of urotensin Ⅱ receptor antagonist on the level of urotensin Ⅱ in plasma and bronchoalveolar lavage fluid in rats with acute lung injury
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 11
- DOI:
- 10.3760/j.issn:1671-0282.2006.11.004
- Key Word:
- 急性肺损伤;血浆;支气管肺泡灌洗液;尾加压素Ⅱ;尾加压素Ⅱ受体拮抗剂
Abstract： 目的 通过测定急性肺损伤(acute lung injury,ALI)血浆及肺泡灌洗液中尾加压素Ⅱ(urotensinⅡ,UⅡ)的含量,来探讨尾加压素受体拮抗剂(UⅡreceptor antagonist,URA)对ALI中UⅡ的影响.方法 Sprague Dawley(SD)大鼠28只,随机分为4组,每组7只:生理盐水对照组(A组);另三组以URA干预油酸型ALI动物模型,分别编为B、C、D组.3 h后4个组均抽动脉血作血气分析,A组、B组抽动脉血后随即经心脏取血各2 ml待测,C组、D组分别于12 h、24 h各取同量心脏血待测.各组均于心脏取血后活杀并取肺,以右肺作支气管肺泡灌洗(bronchoalveolar lavage,BAL),灌洗液(bronchoalveolar lavage fluid,BALF)离心后留取上清液,与心脏血分离的血浆一起在-80℃冻存待测.结果 血气分析显示,B、C、D组血氧分压明显低于A组.A、B、C、D组血浆UⅡ质量浓度分别为(118.1±9.8)pg/ml、(133.6±8.9)pg/ml、(132.0±9.8)pg/ml、(114.0±9.1)pg/ml;相应各组BALF中UⅡ质量浓度分别为(10.1±1.4)pg/ml、(21.5±3.5)pg/ml、(63.1±6.5)pg/ml、(79.0±8.8)pg/ml.ALI大鼠血浆中UⅡ浓度进行性降低,而BALF中UⅡ进行性升高,差异具有统计学意义.结论 UⅡ可能通过活化URA参与ALI的发病过程,并可能对ALI具有某种保护作用.
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