You Position: Home > Paper
Effects of Toll-like receptor 4 in lipopolysaccharide induced acute lung injury
( views:328, downloads:48 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 8
- DOI:
- 10.3760/j.issn:1671-0282.2006.08.006
- Key Word:
- 急性肺损伤;内毒素;Toll样受体4
Abstract： 目的 观察Toll样受体4(toll-like receptor 4,TLR4)基因突变型小鼠与野生型小鼠内毒素攻击致急性肺损伤(acute lung injury,ALI)的差异,分析TLR4在ALI中的作用.方法 采用TLR4基因突变小鼠(C3H/HeJ品系,TLR4-/-)及野生型小鼠(CBA品系,TLR4+/+),用内毒素攻击复制小鼠ALI模型,用显微病理及肺干湿重比(W/D)分析肺损伤程度,检测肺组织髓过氧化物酶(MPO)水平,探讨损伤差异的可能机制.结果 用1,5 mg·kg-1 LPS溶液经尾静脉注射复制ALI模型,结果示TLR4突变小鼠肺组织大体及显微病理损伤较野生型小鼠减轻,同时肺组织水肿(W/D)亦较野生小鼠减轻,尤其是在大剂量(5 mg·kg-1)时,差异有显著性[(4.08±0.1) vs.(4.55±0.2),n=10,t=12.71,P＜0.01].突变小鼠肺组织PMN浸润水平较野生型低[MPO:1 mg·kg-1组,(20.73±4.58) vs.(39.97±3.66),n=10,t=13.43,P＜0.01];5 mg·kg-1组,[(24.0±3.94) vs.(48.5±4.07),n=10,t=15.33,P＜0.01],且两者均较假手术组高.结论 TLR4可能通过介导中性粒细胞肺内聚集而导致ALI的发生发展.
- [1]蔡栩栩,刘春峰,杜悦,等.内毒素致新生和成年大鼠急性肺损伤的比较[J].2005,(6).
- [2]熊旭明,江慧琳,刘炳烦,等.白细胞介素-10对内毒素诱导急性肺损伤大鼠炎症因子的影响[J].2005,(5).
- [3]R B, Yang,M R, Mark,A, Gray,etc.Toll-like receptor-2 mediates lipopolysaccharide-induced cellular signalling.[J].1998,395(6699).
- [4]A, Poltorak,X, He,I, Smirnova,etc.Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene.[J].1998,282.
- [5]Roger G SPRAGG.2003年度急性肺损伤[J].2003,(12).
- [6]O'Neill LA.Therapeutic targeting of Toll-like receptors for inflammatory and infectious diseases.[J].2003,3(4).
- [7]Agorreta J,Garayoa M,Montuenga LM,etc.Effects of acute hypoxia and lipopolysaccharide on nitric oxide synthase-2 expression in acute lung injury.[J].2003,168(3).
- [8]Kinoshita M,Ono S,Mochizuki H.Neutrophils mediate acute lung injury in rabbits: role of neutrophil elastase.[J].2000,32(6).
- [9]Hoyle GW.Neurotrophins and lung disease.[J].2003,14(6).
- [10]Polentarutti N,Vecchi A,Mantovani A,etc.Extracellular and intracellular decoys in the tuning of inflammatory cytokines and Toll-like receptors: the new entry TIR8/SIGIRR.[J].2004,75(5).