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The effects of ammonium perchlorate (AP) on pulmonary fibrosis
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 5
- DOI:
- 10.3760/j.issn:1671-0282.2006.05.006
- Key Word:
- 过氯酸铵;肺纤维化;转化生长因子-β1;肺成纤维细胞
Abstract： 目的研究过氯酸铵(AP)致肺纤维化作用.方法 AP染毒大鼠肺泡巨噬细胞(AM)24h,取AM上清液培养肺成纤维细胞(FB),检测AM转化生长因子-β1(TGF-β1)蛋白表达水平及FB增殖活性、细胞内羟脯氨酸(Hyp)含量.采用气管内注入方式对大鼠进行AP染毒,3 d后处死,测定肺组织中TGF-β1 mRNA的表达.结果 AP低、高剂量组TGF-β1细胞阳性率和AP高剂量组TGF-β1光密度值均显著高于对照组(P＜0.01).各AP染毒组FB增殖活性和FB Hyp含量与对照组比较,差异无显著性(P＞0.05).AP低、中、高剂量组肺组织TGF-β1mRNA表达水平均高于对照组(P＜0.05或P＜0.01).结论 AP增加了AM和肺组织TGF-β1表达,但尚未表现出致肺纤维化作用.
- [1]刘瑞,李成刚,梁欣,等.博莱霉素诱导大鼠肺纤维化过程中羟脯氨酸及氧化应激指标的变化[J].2004,(23).
- [2]张坚松,于风江,瞿树林,等.大鼠肺泡巨噬细胞对人胚肺成纤维细胞增殖的抑制作用[J].2002,(3).
- [3]杨海兵,彭开良,赵素丽,等.过氯酸铵致肺纤维化作用的实验研究[J].2005,(1).
- [4]杨海兵,彭开良,褚启龙,等.过氯酸铵对肺组织损害作用的研究[J].2004,(2).
- [5]张捷,李晓霞,张云霞,等.肺间质纤维化形成中相关细胞因子mRNA表达的实验研究[J].2004,(6).
- [6]Qingjian, Wang,Dallas M, Hyde,Philip J, Gotwals,etc.Effects of delayed treatment with transforming growth factor-beta soluble receptor in a three-dose bleomycin model of lung fibrosis in hamsters.[J].2002,28(6).
- [7]Kelly M,Gauldie J,Kolb M,etc.Re-evaluation of fibrogenic cytokines in lung fibrosis.[J].2003,9(1).
- [8]Reza, Aghanouri,Mostafa, Ghanei,Jafar, Aslani,etc.Fibrogenic cytokine levels in bronchoalveolar lavage aspirates 15 years after exposure to sulfur mustard.[J].2004,287(6).
- [9]Helen M, Crane,Daniel B, Pickford,Thomas H, Hutchinson,etc.Effects of ammonium perchlorate on thyroid function in developing fathead minnows, Pimephales promelas.[J].2005,113(4).
- [10]Deng YM,Yao HW,Tang HF,etc.TGF-beta1 induces alveolar epithelial to mesenchymal transition in vitro.[J].2004,76(1).
- [11]York RG,Barnett J,Girard MF,etc.Refining the effects observed in a developmental neurobehavioral study of ammonium perchlorate administered orally in drinking water to rats. II. Behavioral and neurodevelopment effects.[J].2005,24(6).
- [12]Mitsuru, Machide,Toshikazu, Nakamura.[Transforming growth factor (TGF)].[J].2005,63 Suppl 8.
- [13]Khan MA,Fenton SE,Swank AE,etc.A mixture of ammonium perchlorate and sodium chlorate enhances alterations of the pituitary-thyroid axis caused by the individual chemicals in adult male F344 rats.[J].2005,33(7).
- [14]Rimal B,Greenberg AK,Rom WN.Basic pathogenetic mechanisms in silicosis: current understanding.[J].2005,11(2).