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Effects of mesenchymal stem cells transplantation on post - infarction ventricular remodeling in rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 4
- DOI:
- 10.3760/j.issn:1671-0282.2006.04.006
- Key Word:
- 骨髓间充质干细胞;细胞移植;心室重构;急性心肌梗死
Abstract： 目的探讨骨髓间充质干细胞(mesenchymal stem cells,MSCs)移植对大鼠心肌梗死后心室重构和心功能的影响.方法取大鼠的骨髓,体外分离、扩增培养MSCs,BrdU标记.在结扎冠脉后1～2 h将细胞直接注射到大鼠心脏梗死边缘区.6周后,采用超声心动图和解剖直测法获得大鼠心功能、心室重构和病理资料.结果与对照组比较,细胞移植组左室舒张末期和收缩末期内径缩短,左室前壁增厚,重量指数减小,长径增加,短轴周长缩短(P＜0.05).组织病理发现细胞移植组心梗区心肌数目增加,胶原形成明显减少,血管周围胶原沉积减少,血管密度上调,并在心梗区见到BrdU阳性细胞.结论骨髓间充质干细胞可以在心梗区定植,促进心肌和血管生成,减少胶原形成,从而延缓心肌梗死后心室重构,改善心功能.
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