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Transplantation of adipose stromal cells for treatment of acute myocardial infarction in rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 4
- DOI:
- 10.3760/j.issn:1671-0282.2006.04.005
- Key Word:
- 脂肪基质细胞;移植;急性心肌梗死;大鼠
Abstract： 目的探讨大鼠脂肪基质干细胞(ASCs)移植于梗死心肌后的增殖分化情况及对心功能的影响.方法培养大鼠ASCs,流式细胞术鉴定其表型.将SD大鼠左前降支结扎制造急性心肌梗死模型后,在梗死心肌处植入DAPI标记ASCs(实验组)或DMEM培养液(对照组).移植后1及4周,超声检查心功能.并取梗死区心肌组织进行移植细胞形态学检查及毛细血管密度测定.RT-PCR、ELISA法测梗死区VEGF的mRNA及蛋白表达情况.结果培养获得ASCs,表型为CD44+、CD105+,CD31-、CD45-.植入梗死区的ASCs可以分化为血管内皮细胞,实验组梗死心肌处血管密度较对照组明显增高(P＜0.01),且VEGF基因及蛋白水平在移植后1周均较对照组明显增高(P＜0.01).移植后4周,实验组大鼠左室射血分数(LVEF)及左室短轴缩短率(FS)较对照组明显提高(P＜0.01).结论ASCs移植可以促进大鼠梗死后心肌血管新生,改善心功能.
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