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Effect of cell cycle inhibitor on expression of apoptotic cells in rats after focal cerebral ischemia
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1671-0282.2006.01.004
- Key Word:
- 细胞周期;周期蛋白依赖激酶抑制剂;细胞凋亡;脑缺血
Abstract： 目的探讨细胞周期抑制剂对脑神经细胞凋亡的影响.方法采用光化学方法制作局灶性缺血模型,干预组大鼠腹腔注射细胞周期抑制剂Olomoucine.用HE染色测定皮层梗死灶的体积;免疫组织化学法观察缺血后第3天假手术组、缺血对照组和干预组大鼠梗死灶周围凋亡细胞的表达;用聚丙烯酰胺凝胶电泳(Western blot)观察Caspase-3蛋白的表达.结果对照组梗死灶明显大于干预组;干预组梗死灶周围凋亡细胞数较假手术组显著增高,但明显低于对照组(P＜0.05);Western blot显示Caspase-3蛋白的表达,干预组较假手术组增高(P＜0.05),而较对照组明显降低(P＜0.05).结论脑缺血损伤后细胞周期调控参与了神经细胞的凋亡过程.
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