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Effect of heat shock protein 70 on liver during hemorrhagic shock after trauma
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 11
- DOI:
- 10.3760/j.issn:1671-0282.2005.11.008
- Key Word:
- 创伤;失血性休克;热休克蛋白;肝保护;肝损害
Abstract： 目的探讨热休克蛋白70(HSP70)对严重创伤休克后肝脏作用.方法成年Wistar大鼠,采用双侧股骨骨折伴失血性休克致严重创伤模型,动态观察伤后8 h大鼠肝组织HSP70、血清肝功能生化指标、肝脏病理等变化.HSP70表达测定采用免疫印迹法,并进行计算机图像分析.结果伤后HSP70在肝组织中表达迅速增加,6 h达到峰值,伤后8 h仍维持较高水平;创伤合并休克后,HSP70表达高峰提前至伤后4 h,持续表达至伤后6 h后逐渐下降,死亡前在肝组织中仍有少量表达.创伤休克后血清ALT、TB伤后4 h开始明显增高(P＜0.01),白蛋白下降(P＜0.01).肝脏镜下创伤休克后6 h肝窦内出现较多炎性细胞浸润.结论在创伤休克早期,HSP70可能参与了肝组织细胞抗损伤机制的启动,但随着休克时间的延长,HSP70的过高持续表达,则可能对肝脏造成损害.HSP70在创伤休克后肝保护与肝损害过程中可能发挥双重作用.
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