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Expression of protein kinase C in hemorrhagic shock with acute lung injury
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 10
- DOI:
- No doi available
- Key Word:
- 失血性休克;内毒素;急性肺损伤;蛋白激酶C
Abstract： 目的探讨蛋白激酶C(PKC)在失血性休克并发急性肺损伤动物体内的变化与意义.方法健康成年雄性SD大鼠40只,随机分为对照组,失血性休克组和失血性休克+内毒素组,以大鼠失血性休克时LPS所致ALI为模型,采用酶联免疫吸附(ELISA)法检测血清TNF-α、IL-1β及IL-6,Westernblot检测肺组织TLR4与PKC的蛋白表达,EMSA检测NF-κB活性变化,观察肺脏含水量、肺脏病理变化.结果失血性休克早期即可发生肺血管通透性增高,肺脏含水量上升,组织学检查显示肺脏出现明显的病理损害,肺组织PKC与NF-κB DNA活性、TLR4同步增强,尤以内毒素作用后为著.结论PKC的活化参与了失血性休克时ALI的发病,即TLRs/NF-κB信号通路的活化.
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