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Effects of autologous marrow mesenchymal stem cells transplantation on cardiac function and plasma brain natriuretic peptide in rabbits with dilated cardiomyopathy
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 7
- DOI:
- 10.3760/j.issn:1671-0282.2005.07.010
- Key Word:
- 骨髓;基质干细胞;阿霉素;扩张型心肌病;脑钠肽
Abstract： 目的探索自体骨髓基质干细胞(MSCs)移植对兔扩张型心肌病(DCM)心功能及血浆脑钠肽(BNP)的影响.方法 36只健康大白兔经耳缘静脉注射盐酸阿霉素建立DCM模型后,将存活兔随机分为细胞移植组、对照组和假手术组.移植前和移植后4周,采用超声心动图测定左室收缩末期内径(LVDs)、左室舒张末期内径(LVDd)及左室射血分数(LVEF),并测定血浆BNP水平.移植后4周测定血流动力学指标,免疫荧光检测移植区组织内存活的移植细胞.结果移植后4周移植组的左心室收缩压、压力变化最大速率及LVEF明显高于其他两组(P＜0.01).移植组的LVDd、LVDs和左室舒张末压较假手术组明显减小(P＜0.05、P＜0.01).移植后4周移植组的血浆BNP水平显著下降(P＜0.01),而对照组及假手术组中均无显著性改变;移植组组织切片可见BrdU染色阳性细胞,核发绿色荧光,其他组均未见BrdU染色阳性细胞.结论 MSCs移植能改善阿霉素诱导DCM的心功能,作用机制中神经体液调节可能起重要作用.
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