Effects of pulmonary surfactant on acute lung injury in rats
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 6
- DOI:
- 10.3760/j.issn:1671-0282.2005.06.004
- Key Word:
- 急性肺损伤;肺表面活性物质;大肠杆菌;肺炎;治疗
Abstract： 目的观察外源性肺表面活性物质对大肠杆菌(E.coli)导致的大鼠急性肺损伤(ALI)的作用.方法设立正常大鼠组(N组)和3个实验组[E.coli导致的ALI大鼠PaO2/FiO2＜200mmHg(1mmHg=0.133 kPa)]:对照组(c组,不用药)、表面活性物质组(PS组,经气管插管滴入curosurfR2 ml/kg,160mg/kg)、生理盐水组(NS组,经气管插管滴入NS,2 ml/kg).每30 min检测血气、胸肺有效动态顺应性,180min结束实验,进行肺病理检查,测肺指数、支气管肺泡灌洗液(BALF)蛋白含量和TNF-α浓度.结果①PS组各时点PaO2较给药前升高约70%,与c组、NS组比较明显升高(P＜0.05);②PS组PaCO2、pH以及胸肺有效动态顺应性各时点好于NS组(P＜0.05);③PS组肺指数明显低于C组[(0.38±0.05)%对(0.43±0.05)%,P＜0.05]和NS组[(0.47±0.06)%,P＜0.01];④PS组肺透明膜形成较NS组减少(P＜0.05),较C组有减少趋势(P=0.055);⑤PS组BALF蛋白含量明显低于C组[(0.63±0.30)mg/ml对(0.96±0.25)mg/ml,P＜0.05]和NS组[(1.03±0.27)mg/ml,P＜0.01];TNF-α浓度明显低于NS组[(0.35±0.23)ng/ml对(2.14±1.02)ng/ml,P＜0.01],较C组(0.61±0.28)ng/ml有减低趋势(P＞0.05).结论肺表面活性物质对E.coli所致的大鼠肺内源性ALI有治疗作用,可改善氧合,减轻肺水肿,并有减少肺透明膜形成和TNF-α释放趋势.
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