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Effect of 3-nitropropionic acid preconditioning on the apoptotic cells death an d expression of bcl-2/bax following transient local cerebral ischemia in rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 7
- DOI:
- 10.3760/j.issn:1671-0282.2004.07.009
- Key Word:
- 3-硝基丙酸;脑缺血;细胞凋亡;bcl-2;bax
Abstract： 目的探讨小剂量线粒体毒素3-硝基丙酸(3-nitropropionic aci d,3-NPA)对大鼠局灶性脑缺血神经细胞凋亡和bcl-2/bax表达的影响.方法大鼠腹腔注射3-NPA 20 mg*kg-1或生理盐水后3 d制作大脑中动脉闭塞模型,分别采用TTC染色、流式细胞术、免疫组织化学方法,观察3-N PA预处理对脑缺血2 h再灌注24 h脑梗死体积、神经细胞凋亡率、bcl-2和bax表达的影响.结果 3-NPA预处理组较对照组脑梗死体积减小,神经细胞凋亡减少,bcl-2表达增强,bax表达降低. 结论 3-NPA预处理可以诱导脑缺血耐受,增强bcl-2的表达,降低bax的表达,从而抑制神经细胞凋亡可能是其机制之一.
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