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The effects of glucocorticoid(GC),glucocorticoid receptor(GR),and heat shock protein 70(HSP70) in hepatic injury in the early stage of severe trauma in rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 10
- DOI:
- 10.3760/j.issn:1671-0282.2003.10.005
- Key Word:
- 创伤;受体,糖皮质激素;热休克蛋白类;肝损伤
Abstract： 目的探讨严重创伤后糖皮质激素(GC)、糖皮质激素受体(GR)、热休克蛋白(HSP70)在肝脏继发性损伤中的作用机制.方法成年雄性Wistar大鼠88只,随机分成正常对照组、严重创伤组、阻断50%GR伴严重创伤组.采用严重胸部撞击伤伴单侧股骨骨折模型、动态观察创伤后大鼠血清皮质醇浓度、肝组织GR及HSP70、肝脏病理、血清肝功能生化指标等变化.结果创伤后血浆皮质醇浓度迅速增加,伤后4 h达到峰值,24 h仍维持在高水平;肝组织GR的结合容量伤后即开始下降,12 h降至最低,24 h有所回升,HSP70在伤后增加,8 h达到高峰,24 h仍持续在较高水平;但肝脏病理、血清ALT、TB的改变差异无显著性.使用GR阻断剂后,血浆皮质醇浓度较严重创伤组明显增加,肝淤血明显,肝窦内有较多炎性细胞浸润;血清ALT、TB在伤后早期即有明显升高;肝组织HSP70较单纯创伤组明显增多.结论 GR不足可导致创伤后继发性肝功能不全的发生;HSP70参与了肝组织细胞抗损伤机制的启动;GC、GR及HSP70在严重创伤后肝组织细胞的损伤与抗损伤机制方面起着重要作用.
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