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Effects of nuclear factor -κB activation on acute lung injury
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 9
- DOI:
- 10.3760/j.issn:1671-0282.2003.09.006
- Key Word:
- 急性肺损伤;核因子-κ Β;肿瘤坏死因子α
Abstract： 目的探讨核因子-κΒ(NF-κΒ)活化在炎症反应及其内毒素致伤大鼠急性肺损伤(acute lung injury,ALI)发病中的作用,为临床ALI防治提供理论依据.方法观察内毒素(LPS)对大鼠血气分析和肺组织损伤的影响,采用凝胶电泳迁移率改变(EMSA)法检测肺组织核蛋白提取物中NF-κΒ活性及其特异性;并应用ELISA法检测肺组织匀浆TNFα含量的改变.结果 LPS静注可致大鼠急性肺损伤,肺组织核蛋白的NF-κΒ活性显著增强,肺组织匀浆ΤΝFα含量显著升高.结论 LPS激活NF-κΒ启动ΤΝFα表达,释放的ΤΝFα与LPS进一步共同激活效应细胞的NF-κΒ活化,进而启动一系列参与炎症反应的炎症分子表达而参与大鼠急性肺损伤的发生.
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