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The effect of shengmai injection on the expression of iNOS regulated by protein kinase C
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- Author:
- No author available
- Journal Title:
- JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1671-0282.2003.01.007
- Key Word:
- 生脉注射液;内毒素;诱导性一氧化氮合酶;基因表达
Abstract： 目的探讨生脉注射液对蛋白激酶C调控LPS诱导单核/巨噬细胞iNOS基因表达的影响.方法用蛋白激酶C(protein kinase C,PKC)活性测定法研究LPS对PKC的激活作用及Griess还原法测定NO的生成;用基因重组技术构建iNOS荧光素酶报告基因载体;用基因转染及报告基因检测等方法研究生脉注射液调控LPS刺激RAW264.7细胞对iNOS启动子活性的诱导作用及其与PKC的关系.结果生脉注射液可负调节LPS刺激RAW264.7细胞引起的PKC磷酸化激活和膜移位,并可延长PKC抑制剂H-7下调NO作用时间;荧光素酶表示基因实验结果显示,生脉注射液可抑制LPS刺激诱导的iNOS启动子的转录活性,并可增强H-7和钙离子通道阻滞剂Verapamil作用.结论生脉注射液抑制LPS刺激RAW264.7细胞所致的NO生成增加,其机制之一可能是通过抑制PKC激活和细胞内钙增加而影响iNOS启动子的转录活性.
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