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The role of nuclear factor kappa B activation in rats with acute lung injury
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- Author:
- No author available
- Journal Title:
- JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1671-0282.2003.01.005
- Key Word:
- 急性肺损伤;核因子-κB;细胞因子;内毒素
Abstract： 目的探讨核因子(NF)-κB在内毒素(LPS)诱导的急性肺损伤小鼠发病中的作用.方法腹腔内注射LPS诱导小鼠急性肺损伤模型.LPS注射后0、1、3、6、12测定肺湿重/干重比值(W/D),迁移率改变电泳法检测肺组织NF-κB活性,同时酶联免疫吸附法测定肺组织匀浆中肿瘤坏死因子(TNF)-α、白介素(IL)-10浓度,RT-PCR检测mRNA表达.结果 LPS注射后,W/D比值明显增高,6 h升高最明显(4.82±0.10),显著高于LPS注射前(3.67±1.04,P＜0.05).LPS注射后肺组织核蛋白NF-κB活性明显增强,6 h达到峰值(405.7±62.4),显著高于LPS注射前(44.8±30.9,P＜0.05).肺组织匀浆TNF-α和IL-10浓度分别在LPS注射后6 h和12 h升高最明显,分别为(197.1±52.4)pg/ml和(64.9±39.7)pg/ml,显著高于LPS注射前[分别为(61.2±10.7)pg/ml和(71.6±15.9)pg/ml].与LPS注射前比较,LPS注射后3～12 h,肺组织匀浆TNF-α和IL-10mRNA表达显著增高.肺组织病理显示肺泡出血、水肿、大量炎症细胞浸润,电镜下见Ⅰ型肺泡上皮细胞断裂,Ⅱ型肺泡上皮细胞变性.结论 LPS导致肺组织NF-κB的活化,介导炎症介质大量表达,参与急性肺损伤发生.
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