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Mechanism of bone regeneration promoted by calcium alginate film

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Author:: No author available

Journal Title:: JOURNAL OF EMERGENCY MEDICINE

Issue:: 6

DOI:: 10.3760/j.issn:1671-0282.2002.06.007

Key Word:: 骨损伤;海藻酸钙膜;引导性骨再生;骨诱导因子

Abstract：目的研究海藻酸钙膜引导骨缺损再生的有效性和优越性,进一步探讨其成骨机制.方法以家兔为研究对象,在其双侧胫骨形成洞形骨缺损,实验侧骨缺损上覆盖海藻酸钙膜,对照侧未覆盖任何膜,分别在术后1、2、4、6、8周时取出胫骨,进行大体观察、X线观察、组织学光镜观察、电镜观察以及TGF-β、VEGF免疫组化染色.结果海藻酸钙膜4～6周吸收;实验侧术后4周X线观察可见骨密度达到正常皮质骨,对照侧6周才达到;前者大体观察骨缺损愈合表面平整,后者表面不连续;实验侧术后2周光镜下可见骨小梁构成编织骨,对照侧4周可见;电镜下术后6周实验侧骨痂致密,对照侧骨痂疏松.TGF-β、VEGF免疫组化表达则有其成骨作用的时空性.结论海斟酸钙膜通过对骨诱导因子的早期调控引导骨再生,效果良好,能更快地促进骨损伤修复,且6周降解不影响骨愈合再生.

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