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The effects of L-Tetrahydropalmatine on the expression of Bcl-2 and Bax in rats after acute global cerebral ischemia and reperfusion
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- Author:
- No author available
- Journal Title:
- JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 6
- DOI:
- 10.3760/j.issn:1671-0282.2002.06.006
- Key Word:
- 脑缺血再灌注;神经元凋亡;左旋四氢巴马汀;Bcl-2;Bax
Abstract： 目的观察左旋四氢巴马汀(L-tetrahydropalmatine,L-THP)在脑缺血再灌注时对凋亡相关基因Bcl-2、Bax蛋白表达的影响.方法建立大鼠急性全脑缺血再灌注模型,采用免疫组织化学法、原位末端标记法,分别检测假手术组(S组)、缺血再灌注组(Ⅰ组)、L-THP治疗组(T组)海马CA1区Bcl-2、Bax蛋白表达水平及凋亡细胞数.结果 (1)脑缺血再灌注时,海马CA1区Bcl-2蛋白的表达显著增加(P＜0.01),但随时间的延长,增加幅度逐渐减小;而T组Bcl-2蛋白表达于相应的时间点则明显大于I组(P＜0.01).(2)I组各时间点Bax蛋白表达均大于S组(P＜0.01),且随时间延长增加幅度逐渐增大;而T组Bax蛋白表达在相应时间点则下降(P＜0.01).(3)T组的神经细胞凋亡数与Bcl-2/Bax蛋白阳性细胞数比值的关系为负相关:r＝-0.94173,P＜0.001.(4)神经细胞凋亡数随再灌注时间延长而增加,L-THP可减少细胞凋亡数.结论在脑缺血再灌注细胞凋亡过程中,L-THP可通过上调Bcl-2蛋白表达,下调Bax蛋白表达,减少神经元凋亡,对脑缺血损伤起保护作用.
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