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The effect of recombinant bactericidal/permeability increasing protein on tissue tumor necrosis factor-α expression in septic rats
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- Author:
- No author available
- Journal Title:
- JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 3
- DOI:
- 10.3760/j.issn:1671-0282.2002.03.007
- Key Word:
- 杀菌/通透性增加蛋白;肿瘤坏死因子-α;脓毒症;多器官功能障碍综合征
Abstract： 目的探讨杀菌/通透性增加蛋白(BPI)对脓毒症动物肝、肺肾组织肿瘤坏死因子-α(TNF-α)表达及多器官功能障碍的影响与意义.方法采用大鼠盲肠结扎穿孔(CLP)造成脓毒症模型.动物分为正常对照组(10只)、CLP组(20只)及BPI治疗组(20只).CLP后12h和24h处死动物,分别检测肝、肺、肾组织TNF-α mRNA表达、TNF-α蛋白水平和器官功能指标.结果 CLP后12h动物肝、肺、肾组织TNF-α mRNA表达均显著增强,分别为伤前基础值的2.46倍(P＜0.05)、2.86倍(P＜0.01)、2.27倍(P＜0.01),同时各组织TNF-α水平迅速升高(P＜0.01).重组BPI治疗可不同程度地抑制肝、肺、肾组织TNF-α mRNA表达上调(P＜0.05),12h各组织TNF-α水平显著降低(P＜0.05),均恢复至伤前正常范围.此外,BPI组动物血清丙氨酸转胺酶、肌酐水平在CLP后12h显著低于未治疗组,CLP后24h肺组织髓过氧化物酶活性也明显下降(P＜0.01).结论严重腹腔感染早期应用重组BPI治疗有助于下调TNF-α的过量表达,从而抑制机体过度炎症反应,防止多器官功能障碍的发生与发展.
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