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Acute hepatocytes injury caused by APAP and effects of verapamll DU Xiaodong,
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1674-5760.2001.04.001
- Key Word:
- 醋氨酚/中毒;肝损伤;维拉帕米
Abstract： 目的通过观察[Ca2+]i、MDA、SOD、LDH在急性醋氨酚(APAP)致肝损伤中的变化,探讨APAP致肝损伤机制,并且观察维拉帕米预处理是否可以预防.方法采用两步灌流法分离肝细胞进行原代培养,观察加入10mmol/LAPAP后30min、1h、2h、4h、8h各时点[Ca2+]i、MDA、SOD、LDH的变化;另在加入APAP前2h用不同浓度(20、40、80μg/ml)的维拉帕米预处理,4h后观察上述指标的变化.结果在10mmol/L APAP处理后,30min出现[Ca2+]i升高,1h后出现MDA升高,SOD和LDH在第2～4h升高(以上均P＜0.05);终浓度为20μg/ml、40μg/ml的维拉帕米预处理明显使各值下降(P＜0.05),而80μg/ml使各数值不变或上升.结论[Ca2+]i的显著性升高是急性醋氨酚中毒肝损伤的始动因素;一定浓度的维拉帕米预处理对这种肝损伤有保护作用.
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