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EST-SSR markers in Agrocybe cylindracea and its application
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- Author:
- No author available
- Journal Title:
- Mycosystema
- Issue:
- 5
- DOI:
- No doi available
- Key Word:
- 柱状田头菇;短片段重复;分子标记;菌株特征
Abstract： 借鉴香菇EST序列设计的40对引物对柱状田头菇进行了EST-SSR引物通用性研究.结果显示,挑选的28对引物中多态性好且稳定的有13对,通用率达46.4％.PCR扩增产物获得具有多态性条带81条,每对引物扩增条带的数目范围为2-14,平均5.86条,扩增片段大小在100-400bp之间.多态信息含量(PIC)在0.3750-0.8032,平均0.7084.研究结果表明,EST-SSR分子标记在食用菌的遗传多样性和比较基因组学研究中起到重要的作用.
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