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Molecular mechanism of the interaction between amanitin and RNA polymerase Ⅱ
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- Author:
- No author available
- Journal Title:
- Mycosystema
- Issue:
- 5
- DOI:
- No doi available
- Key Word:
- 鹅膏菌;鹅膏毒肽;RNA聚合酶Ⅱ;分子对接
Abstract： 研究鹅膏毒肽与RNA聚合酶Ⅱ相互作用的分子机制,利用分子对接方法获得了9种鹅膏毒肽与RNA聚合酶Ⅱ相互作用的结合模式、结合位点、对接能和抑制常数等信息,并对鹅膏毒肽的毒性与结构间的构效关系进行了考察.结果表明:利用分子对接方法获得的鹅膏毒肽与RNA聚合酶Ⅱ相互作用的信息与实验结果相一致;不同R2取代基引起毒素与聚合酶Ⅱ结合能力强弱不同,从而导致鹅膏毒肽分子间的毒性差异.结果证实了运用分子对接方法探索多肽分子与蛋白质相互作用的可行性,为在分子水平上研究多肽与蛋白质的相互作用开拓了新的思路.
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