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Transcriptional analysis of different glycoside hydrolase genes in Trichoderma reesei
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- Author:
- No author available
- Journal Title:
- Mycosystema
- Issue:
- 5
- DOI:
- No doi available
- Key Word:
- 转录组测序;纤维素酶;实时荧光定量PCR;α-甘露糖苷酶
Abstract： 以前期里氏木霉RNA-seq中发现的7个糖苷水解酶基因为对象,分析其不同条件下的表达特性,以期为寻找新的纤维素降解功能酶提供证据.运用生物信息学方法,分析了7个基因可能的编码产物和结构特征.以不同的产纤维素酶菌株(QM 9414、RUT C30)为材料,采用实时荧光定量PCR,对7个糖苷水解酶基因(编号4-10)在各种碳源条件下转录情况与主要的3个纤维素酶基因cbh1,cbh2,egl1(编号1-3)进行了比较分析.信息学分析表明,7个基因编码蛋白分属于GH47(4号、5号),GH92(6-8号),GH16(9号),GH31(10号)糖苷水解酶家族,具有典型的信号肽序列.cbh1,cbh2,egl1基因在纤维素酶诱导条件下,转录水平均表现显著的增加,上调倍数以QM 9414菌株表现的最高.QM 9414菌株中,cbh1,cbh2,egl1基因在纤维素条件下的上调倍数显著高于乳糖,3个基因在RUT C30菌株中的转录水平则显示乳糖条件下上调幅度更大.7个糖苷水解酶基因也存在类似的情况,而且编码α-甘露糖苷酶和内切β-葡聚糖酶的8号、9号基因上调倍数在纤维素酶诱导条件下仅次于纤维素酶基因,而以甘油为碳源条件下,8号、9号基因上调倍数高于纤维素酶基因.4号基因在上述碳源条件下,转录水平变化不大.结果表明:4号基因可能是组成型表达.基因5、6、7、8、9、10的表达呈现明显的菌株和碳源依赖性,且在纤维素酶诱导条件下基本上是和3个纤维素酶基因共转录的.
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