Levels of NO2-, LTB4, PGE2, IL-6, IL-10 in exhaled breath condensate with acute exacerbation chronic obstructive pulmonary disease patients
- Author:
- No author available
- Journal Title:
- International Journal of Respiration
- Issue:
- 19
- DOI:
- 10.3760/cma.j.issn.1673-436X.2012.019.002
- Key Word:
- 慢性阻塞性肺疾病;呼出气冷凝液;亚硝酸盐;白三烯B4;前列腺素E2;白介素6;白介素10;气道炎症;Chronic obstructive pulmonary disease;Exhaled breath condensate;Nitrate;Leukotriene B4;Prostaglandin E2;Interleukin-6;Interleukin-10;Airway inflammation
Abstract： Objective To investigate the relationship between NO2-,LTB4,PGE2,IL-6 and IL-10levels in exhaled breath condensate (EBC) and airway inflammation as well as pulmonary function from COPD patients.Methods Twenty cases of COPD and twenty healthy control were enrolled.ALL subjects detected FEV1/FVC and FEV1％ predict.EBC were collected using EcoScreen system.The NO2-levei in EBC was determined by the spectrophotometry.The levels of LTB4,PGE2,IL-6 and IL-10in EBC were measured by enzyme linked immuosorbent assay.Results The levels of NO2-and LTB4 in EBC of COPDgroup were significantly higher than that of the control group [(2.029 ± 1.992) μmol/L,(13.598±3.910)ng/L vs (0.400 ±0.235) μmol/L,(9.742 ±2.348) ng/L,respectively,all P ＜0.05].There were no signficant difference of PGE2,IL-6 and IL-10 levels in EBC both groups (all P ＞0.05).There were no correlation between NO2-,LTB4,PGE2,IL-6,IL-10 in EBC with FEV1/FVC and FEV1％ predict (P ＞0.05).Conclusions The COPD patients NO2-and LTB4 levels in EBC are related to airway inflammation and oxidative stress.Whether PGE2,IL-6,IL-10 reflecting airway inflammation need further study.All of cytokines were failed to correlate with Lung functiom.
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