Abstract： Objective To analyze the effect of stratified nursing care on internal medicine hospitalized patients.Methods Patients in the department of internal medicine were treated with stratified nursing care after 4 days in hospital,the sleep status,quality of life and mood differences before and after implementation were compared.Results After nursing care was implemented,the score of sleep quality was 0.93 ± 0.18,sleep disturbance scored 0.63 ± 0.21,daytime function scored 0.47 ± 0.16; SAS scored 40.18 ± 4.92,SDS scored37.72 ± 5.26; QQL scored 198.33 ± 21.49.The sleep status,negative emotion and life quality of patients were improved,the difference was statistically significant ( P ＜ 0.05 ).Conclusions Humanistic nursing concept should be blended into the routine nursing service,it can improve the quality of nursing.