Abstract： Objective To investigate the feasibility of collecting blood specimen from umbilical vein catheter for blood determination.Methods 80 premature infants that indwelling catheter were divided into drawing blood and non-drawing blood groups,then the interrelated complicating disease and adverse reaction between the two groups were compared.First blood was drawn in definite quantity from 40 premature infants in the drawing blood group 30s after suspending transfusion,then blood specimen was collected(observational group one 2ml,observational group two 4ml).While blood specimen in control group was collected by puncturing peripheral blood vessel.At last the blood routine test,biochemistry and liver function in the three groups were tested to analyze and compare the results.Results There was no statistical significance in the discrepancy of the test results among the three groups.There was no significance in the discrepancy of the incidence rate of umbilical venous catheter complication between drawing blood group and non-drawing blood group(P＞0.05).Conclusions The method of drawing 2ml blood 30s after suspending transfusion from umbilical venous catheter and then exsanguinating to test has no obvious infection on the result.Collecting blood from umbilical venous catheter has no obvious infection on regular service of the catheter and it possesses the advantage of being no complaints,high success rate and exsanguinating repeatedly.So it can be used one ofthe way to collect blood specimen.