The Effect of 58S Bioactive Glass Coating on Polyethylene Terephthalates in Graft-Bone Healing
- Author:
- No author available
- Journal Title:
- Journal of Bionic Engineering
- Issue:
- 4
- DOI:
- 10.1016/S1672-6529(11)60133-9
- Key Word:
- anterior cruciate ligament;polyethylene terephthalates;artificial ligament;coating;bioactive glass
Abstract： In this study the effects of surface modification of Polyethylene Terephthalates (PET) fibers with 58S bioactive glasses on osteoblasts proliferation and osseointegration in the tibia-articular tendon-bone healing model were investigated.PET sheets were coated with 58S bioactive glass and uncoated PET sheets were used as a control.Scanning Electron Microscope (SEM) and X-ray photoelectron spectrometer were adopted to analyze the surface characteristics of the fibers.MT3T3-E 1 cells were cultured with the PET fibers and the MTT and ALP were tested at 1,3,5 days.Twenty-four skeletally mature male New Zealand white rabbits were randomly divided into two groups,the 58S-PET group and the PET group.Both groups underwent a surgical procedure to establish a tibia-articular tendon-bone healing model.Mechanical examinations and histological assays were taken to verify the coating effects in vivo.Results of both MTT and ALP tests show significant differences (P ＜ 0.01) between the 58S-PET group and the PET group.At 6 weeks and 12 weeks,the max load-to-failure was significantly higher in the 58S-PET group.In the histological assays,distinct new bone formation was observed only in the 58S-PET group and stronger osseointegration was seen in the 58S-PET group than that in the control group.The 58S-coating on PET could enhance the proliferation and activity of the osteoblasts and therefore promote the new bone formation and tendon-bone healing.
- [1]E E, Berg,M E, Pollard,Q, Kang.Interarticular bone tunnel healing.[J].2001,17(2).
- [2]M, Yamamoto,K, Kato,Y, Ikada.Ultrastructure of the interface between cultured osteoblasts and surface-modified polymer substrates.[J].1997,37(1).
- [3]Q.Z. Chen,A.R. Boccaccini,V. Maquet,etc.Bioactive coatings by vaterite deposition on polymer substrates of different composition and morphology[J].2007,27(4).
- [4]Kawamura S,Ying L,Kim HJ,etc.Macrophages accumulate in the early phase of tendon-bone healing.[J].2005,23(6).
- [5]Gautier S,Boccaccini AR,Hench LL,etc.Development and in vitro characterisation of novel bioresorbable and bioactive composite materials based on polylactide foams and Bioglass for tissue engineering applications.[J].2002,23(18).
- [6]LL Hench,J Wilson.Surface-active biomaterials[J].1984,226 (4675).
- [7]M R, Filgueiras,G, La Torre,L L, Hench.Solution effects on the surface reactions of a bioactive glass.[J].1993,27(4).
- [8]Gupta B,Bisson I,Hilborn J,etc.Acrylic acid grafting and collagen immobilization on poly(ethylene terephthalate) surfaces for adherence and growth of human bladder smooth muscle cells.[J].2002,23(15).
- [9]Larry L. Hench.Genetic design of bioactive glass[J].2009,29(7).
- [10]Kim HJ,Dynybil C,Kawamura S,etc.Tendon healing in a bone tunnel differs at the tunnel entrance versus the tunnel exit: an effect of graft-tunnel motion?[J].2006,34(11).
- [11]Ross Henshaw Sheela George,Jo A. Hannafin,Erik Attia Haydee Brown.Patterns of Gene Expression in a Rabbit Partial Anterior Cruciate Ligament Transection ModelThe Potential Role of Mechanical Forces[J].2010,38(2).
- [12]Nau T,Duval N,Lavoie P.A new generation of artificial ligaments in reconstruction of the anterior cruciate ligament. Two-year follow-up of a randomised trial.[J].2002,84(3).
- [13]F.C. Loh,K.L. Tan,E.T. Kang,etc.Near-u.v. radiation induced surface graft copolymerization of some O3-pretreated conventional polymer films[J].1995,31(5).
- [14]Dong Q,Li L,Wang L,etc.Anterior cruciate ligament reconstruction with LARS artificial ligament: a multicenter study with 3- to 5-year follow-up.[J].2010,26(4).
- [15]L. L. Hench,H. A. Paschall.Direct chemical bond of bioactive glass-ceramic materials to bone and muscle[J].1973,7(3).
- [16]O H, Andersson,I, Kangasniemi.Calcium phosphate formation at the surface of bioactive glass in vitro.[J].1991,25(8).
- [17]Kurt P. Spindler,Rick W. Wright.Anterior Cruciate Ligament Tear[J].2008,359(20).
- [18]Ranstam J,Roos HP,Lohmander LS,etc.A randomized trial of treatment for acute anterior cruciate ligament tears.[J].2010,363(4).
- [19].Antibacterial effect of bioactive glasses on clinically important anaerobic bacteria in vitro[J].2008,19(2).
- [20]Legnani C,Borgo E,Albisetti W,etc.Synthetic grafts for anterior cruciate ligament rupture: 19-year outcome study.[J].2010,17(2).
- [21]Mucalo, MR,Kato, K,Yokogawa, Y.Phosphorylated, cellulose-based substrates as potential adsorbents for bone morphogenetic proteins in biomedical applications: A protein adsorption screening study using cytochrome C as a bone morphogenetic protein mimic[J].2009,71(1).
- [22]Eves B,Frondoza CG,Au AY,etc.Consil bioactive glass particles enhance osteoblast proliferation and maintain extracellular matrix production in vitro.[J].2008,86(3).