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Protein kinase C mediates thrombin-induced monocyte chemoattractant protein-1 release from human lung fibroblasts
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- Author:
- No author available
- Journal Title:
- Journal of Southern Medical University
- Issue:
- 9
- DOI:
- 10.3969/j.issn.1673-4254.2012.09.007
- Key Word:
- 凝血酶;蛋白激酶C抑制剂;单核细胞趋化蛋白-1;人肺成纤维细胞
Abstract： 目的 研究蛋白激酶C(PKC)在凝血酶诱导人肺成纤维细胞(HLF-1)单核细胞趋化蛋白-1(MCP-1)表达信号通路中的作用.方法 分别用几种不同类型的PKC抑制剂预处理HLF-1,再用凝血酶(10nmol/L)刺激HLF-1,利用ELISA法检测上清液中MCP-1的蛋白表达；提取细胞裂解液中总RNA并逆转录合成cDNA,利用实时荧光定量PCR检测MCP-1 mRNA表达水平.结果 广谱型PKC抑制剂Bisindolylmaleimide I和RO-31-8220可抑制凝血酶诱导的HLF-1 MCP-1蛋白释放及mRNA表达,而Ca2+依赖性PKC抑制剂Gǒ 6976则没有抑制效果.结论 非Ca2+依赖性PKC介导凝血酶诱导HLF -1释放MCP-1.
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