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Molecular cloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus

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Author:
No author available
Journal Title:
Zoological Research
Issue:
4
DOI:
10.3724/SP.J.1141.2012.E03-04E40
Key Word:
GIFT tilapia;FTZ-F1;Cloning;Expression

Abstract: The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia (gfFTZ-F1).The full-length cDNA was isolated from the ovary,which included an open reading frame encoding a predicted protein of 486 amino acids.Sequence,tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues.Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts,though further study is needed to make any definitive conclusions.

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