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Phylogenetic analysis and expression patterns of tropomyosin in amphioxus

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Author:: No author available

Journal Title:: Zoological Research

Issue:: 4

DOI:: 10.3724/SP.J.1141.2012.04389

Key Word:: 文昌鱼;进化分析;胚胎发育;表达图式;Tropomyosin

Abstract： Tropomyosin是一种分布广泛而且在进化上十分保守的蛋白,是肌肉形成和收缩过程中重要的调节蛋白质.通过RT-PCR和RACE技术得到文昌鱼tropomyosin基因全长,编码一个含284个氨基酸残基的蛋白质,将文昌鱼Tropomyosin和在其他物种中的同源物进行比对建树,发现其在功能域上高度保守并且只有一个拷贝,符合动物分类学中各物种的进化地位.胚胎整体原位杂交实验得知,tropomyosin在文昌鱼早期发育的表达,最早从原肠胚末期神经胚早期开始,定位于分化中的中内胚层.到神经胚期,tropomyosin的表达出现在发育中的体节和脊索中.随着发育的进行,tropomyosin的表达稳定地集中在体节、脊索处.到72 h幼虫阶段,tropomyosin的表达仍然在肌节内.成体的切片原位杂交结果显示,tropomyosin在肌节中的表达大幅度下调,而在神经管细胞、脊索和腮区腮瓣处仍然可以检测到明显的表达,在外胚层和表皮内没有发现杂交信号.研究结果表明,tropomyosin的表达与文昌鱼肌节、肌肉以及神经索的发生相关,参与文昌鱼胚胎躯体模式的构建,而且在成体的生命活动中发挥重要作用.

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