You Position: Home > Paper
Cloning and expression of Tssk1 & Tssk2 in mice and the presence & localization of them in mature sperm
( views:162, downloads:0 )
- Author:
- No author available
- Journal Title:
- Zoological Research
- Issue:
- 4
- DOI:
- 10.3724/SP.J.1141.2012.04381
- Key Word:
- Tssk蛋白激酶;睾丸特异性;精子功能;磷酸化
Abstract： 睾丸特异性丝氨酸/苏氨酸蛋白激酶(Testis-Specific Serine/Threonine Kinases,TSSKs)可能在精子发生和(或)精子功能调节中起着重要作用,该文研究克隆并表达了小鼠Tssk1和Tssk2基因,纯化得到了Tssk1和Tssk2蛋白激酶,经Western blotting分析,Tssk1和Tssk2皆存在于小鼠和人的成熟精子中.免疫组化的结果显示,Tssk1分布于小鼠的头部(顶体)及整个尾部,Tssk2主要分布在小鼠精子头部顶体后的区域；获能前后的小鼠精子其Tssk1及Tssk2分布模式未发生改变,小鼠Tssk2在诱发顶体反应前后精子中的分布模式也无变化.然而,原来存在于顶体的Tssk1在诱发顶体反应后由于顶体的丢失而未能检出其信号,但尾部的信号不受影响.在人精子中,Tssk1分布区域为颈部及尾部,Tssk2则分布于赤道板的位置.研究结果提示,Tssk1和Tssk2可能对精子功能具有重要调节作用.
- [1]Tanaka T,Toshima J,Mizuno K.Dual specificity protein kinase activity of testis-specific protein kinase 1 and its regulation by autophosphorylation of serine-215 within the activation loop.[J].1999,274(17).
- [2]P E, Visconti,J L, Bailey,G D, Moore,etc.Capacitation of mouse spermatozoa. I. Correlation between the capacitation state and protein tyrosine phosphorylation.[J].1995,121(4).
- [3]杨增明, 孙青原, 夏国良主编.生殖生物学[M].2005.
- [4]Grootegoed JA,van Eenennaam H,van Cappellen WA,etc.Functional transformation of the chromatoid body in mouse spermatids requires testis-specific serine/threonine kinases.[J].2010,123(3).
- [5]Jiro Toshima,Takehiko Koji,Kensaku Mizuno.Stage-Specific Expression of Testis-Specific Protein Kinase 1 (TESK1) in Rat Spermatogenic Cells[J].1998,249(1).
- [6]Xu B,Jha KN,Hao Z.Targeted deletion of Tssk1 and 2 causes male infertility due to haploinsufficiency.[J].2008,319(2).
- [7]Sarah Shalom,Jeremy Don.Tlk, a novel evolutionarily conserved murine serine threonine kinase, encodes multiple testis transcripts[J].1999,52(4).
- [8]Pack SD,Zerfas PM,Starost MF,etc.Identification and characterization of SSTK, a serine/threonine protein kinase essential for male fertility[J].2005,25(10).
- [9]S, Nayak,N, Galili,C A, Buck.Immunohistochemical analysis of the expression of two serine-threonine kinases in the maturing mouse testis.[J].1998,74(1-2).
- [10]Jha KN,Salicioni AM,Arcelay E,etc.Evidence for the involvement of proline-directed serine/threonine phosphorylation in sperm capacitation.[J].2006,12(12).
- [11]W. Bielke,R.J. Blaschke,G.C. Miescher,etc.Characterization of a novel murine testis-specific serine/threonine kinase[J].1994,139(2).
- [12]Gisela Zuercher,Valeria Rohrbach,Anne-Catherine Andres,etc.A novel member of the testis specific serine kinase family, tssk-3, expressed in the Leydig cells of sexually mature mice[J].2000,93(1-2).
- [13]Kopf GS,Hao Z,Visconti PE,etc.Cloning and chromosomal localization of a gene encoding a novel serine/threonine kinase belonging to the subfamily of testis-specific kinases.[J].2001,77(3).
- [14]Xu B,Jha KN,Hao Z.TSKS concentrates in spermatid centrioles during flagellogenesis.[J].2008,319(2).
- [15]Salicioni AM,Bates TC,Visconti PE,etc.Expression and localization of five members of the testis-specific serine kinase (Tssk) family in mouse and human sperm and testis.[J].2011,17(1).
- [16]TA-CHIEN TSENG,SHEAU-HU CHEN,YUN-PUNG PAUL HSU,etc.Protein Kinase Profile of Sperm and Eggs: Cloning and Characterization of Two Novel Testis-Specific Protein Kinases (AIE1, AIE2) Related to Yeast and Fly Chromosome Segregation Regulators[J].1998,17(10).
- [17]P D Walden.A novel 205-kilodalton testis-specific serine/threonine protein kinase associated with microtubules of the spermatid manchette.[J].1993,13(12).
- [18]N B, Hecht.Post-meiotic gene expression during spermatogenesis.[J].1988,267.
- [19]Rohrbach V.,Hemphill A.,Boehlen D. Zuercher G.,etc.A Novel Family of Serine/Threonine Kinases Participating in Spermiogenesis[J].1997,139(7).
- [20]P, Sassone-Corsi.Transcriptional checkpoints determining the fate of male germ cells.[J].1997,88(2).
- [21]L E, Hake,A A, Alcivar,N B, Hecht.Changes in mRNA length accompany translational regulation of the somatic and testis-specific cytochrome c genes during spermatogenesis in the mouse.[J].1990,110.
- [22]Johnson LN.The regulation of protein phosphorylation.[J].2009,37(4).
- [23]Nikolay A. Spiridonov,Julian Sosnik,Patricia V. Miranda.Tssk6 is required for Izumo relocalization and gamete fusion in the mouse[J].2009,122(15).
- [24]Zhang S,Yang Y,Deng Y,etc.Mutation screening and association study of the TSSK4 Gene in Chinese infertile men with impaired spermatogenesis.[J].2008,29(4).
- [25]Xiujuan Chen,Gang Lin,Youheng Wei,etc.TSSK5, a novel member of the testis-specific serine/threonine kinase family, phosphorylates CREB at Ser-133, and stimulates the CRE/CREB responsive pathway[J].2005,333(3).