Myocardial protective effects of urethane on isolated rat heart in prolonged hypothermic preservation

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Author:
WU Tie-Jun(Xijing Hospital,Fourth Military Medical University)
BI Sheng-Hui(Xijing Hospital,Fourth Military Medical University)
CUI Qin(Xijing Hospital,Fourth Military Medical University)
JIN Zhen-Xiao(Xijing Hospital,Fourth Military Medical University)
YI Ding-Hua(Xijing Hospital,Fourth Military Medical University)
LIU Wei-Yong(Xijing Hospital,Fourth Military Medical University)
Journal Title:
JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
Issue:
Volume 30, Issue 24, 2009
DOI:
Key Word:
urethane;organ preservation;heart transplantation

Abstract: AIM:To observe the protective effects of urethane plus HTK on the isolated rat hearts in prolonged hypothermic preservation.METHODS:Male Sprague-Dawley rats(250-280 g)were divided into 2 groups(n=6 per group)according to each preservation solution used:control group,HTK solution;experimental group,HTK solution plus urethane.The hearts were arrested and stored in each preservation solutions respectively for 6 and 18 h at 4℃.After preservation,the myocardial function,ATP content,cardiomyocyte apoptosis and uhrastrueture were examined.RESULTS:Compared with control group,experimental group showed higher reeovery of cardiac.function,lower rate of cardiomyocyte apoptosis and better cardiomyocyte uhrastructure both for 6 and 18 h of preservation.The ATP content after 18 h of preservation in control group was significantly higher than that in experimental group.CONCLUSION:Urethane led to significandy better and longer preservation in isolated hearts subjected to prolonged hypothermic preservation.

  • [1]Masters TN,Fokin AA,Schaper J,et al.Changes in the preserved heart that limit the length of preservation[J].J Heart Lung Transplant,2002,21(5):590.
  • [2]孙长滨,侯芝奇,刘萌萌,等.牛磺酸对低温保存人鼠心脏的心肌形态和漏出酶的影响[J].心脏杂志,2008,20(2):148-149.
  • [3]顾春虎,易定华,王云雅,等.异氟醚预处理对体外循环后心肌的保护作用[J].第四军医人学学报,2008,29(13):1166-1168.
  • [4]Kotanidou A,Choi AMK,Winchurch RA,et al.Urethan anesthesia Droteets rats against lethal endotoxemia and reduces TNF-α release[J].J Appl Physiol,1996,81(5):2304-2309.
  • [5]Tanja P,Giorgio D,Vittorio G.Experimental urethane anaesthesia preyents digoxin intoxication:electrocardiographic and histological study in rabbit[J].Pharmaeol Res,2000,42(4):355-362.
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