Anti-tumor effects of mouse embryonic fibroblasts-mediated interleukin-12(IL-12)on Sarcoma 180 xenografts

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Author:
SUN Yun-Fang(Shandong Medical College)
Journal Title:
JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
Issue:
Volume 30, Issue 24, 2009
DOI:
Key Word:
IL-12;fibroblasts;Sarcoma-180 cancer cells;tumor xenografts

Abstract: AIM:To investigate the anti-tumor effects of mouse embryonic fibroblasts.mediated interleukin-12(IL-12)on Sarcoma 180 xenografts.METHODS:The murine normal fibroblast were transfected with human IL-12 gene(FB/IL-12)by PEI.Controls were set up at the same time.The S-180 cells were inoculated in mice subcutaneously.Then FB/IL-12 was injected in mice in the same place subcutaneously at intervals,and controls were set up.On the 21 st day.tumor Weight was recorded.The serum levels of IL-12 and the expression of interferon-γ(IFN-γ)in mice were detectedby EILISA.The activity of the natural killer cells(NK)and spleen lymphocyte were detected;the percentages of CD4~+/IFN-γ and CD8~+/IFN-γ double positive cells were also investigated.RESULTS:The proliferation inhibition rate of FB/IL-12 group was higherthanthose of the controls(P<0.01).The activity of NK cells and proliferation of Lymphocytes,the serum levels of IL-12 and IFN-γ,the frequency of CD4~+/IFN-γ and CD8~+/IFN-γ double positive cells were also hiigher than controls(P< 0.01).CONCLUSION:By intensifying its immune ability,the mouse embryonic fibroblasts-mediated intedeukin-12(IL-12)on Sarcoma 180 xenografts has better anti-tumor effects.

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