Mechanisms of group Ⅲ mGluRs against cultured neuron apoptosis induced by β-amyloid peptide 31-35
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 24
- DOI:
- 10.3321/j.issn:1000-2790.2009.24.009
- Key Word:
- AB 31-35;caspases活性;第Ⅲ组mGluRs;Aβ31-35;caspases activity;group Ⅲm GluRs
Abstract： AIM:To determine the mechanisms of neuroprotection by group Ⅲ metabotropic glutamate receptors(mGluRs)activation against apoptosis induced by 13-amyloid peptide 31-35 (Aβ31-35)in frontal lobe cortical neurons.METHODS:The cerebral cortical neurons were incubated with A1331-35(25 μmol/L)or coapplied with group Ⅲ mGluRs agonist of L-SOP or(R,S)PPG(100μmol/L)for 24 h.The apoptosis of the cells was analyzed by flow cytometry and easpases activity.RESULTS:L-SOP or(R,S)-PPG(100 μmol/L)significantly inhibited the neuron apoptosis and decreased caepase-3,-8 and-9 in the Aμ31-35 treated neurons.CONCLUSION:The results described in the present study suggested that activation of group Ⅲ mGluRs protected neurons from Aβ31-35-induced apoptosis by blocking the caspase-dependent intrinsic and extrinsic apoptotic pathways.
- [1]Flavell R,Greenberg ME,Morishima Y,etc.Beta-amyloid induces neuronal apoptosis via a mechanism that involves the c-Jun N-terminal kinase pathway and the induction of Fas ligand.[J].2001,21(19).
- [2]Croucher MJ,Vernon AC,Dexter DT.Additive neuroprotection by metabotropic glutamate receptor subtype-selective ligands in a rat Parkinson's model.[J].2008,19(4).
- [3]Awasthi A,Yamada T,Matsunaga Y.Amyloid-beta causes apoptosis of neuronal cells via caspase cascade, which can be prevented by amyloid-beta-derived short peptides.[J].2005,196(2).
- [4]Giardina B,Nocca G,Misiti F,etc.Methionine 35 oxidation reduces toxic and pro-apoptotic effects of the amyloid beta-protein fragment (31-35) on isolated brain mitochondria.[J].2004,126(2).
- [5]Harrington WJ Jr,Schultz DR.Apoptosis: programmed cell death at a molecular level.[J].2003,32(6).
- [6]赵丽,李灵敏,张策.激活第Ⅲ组代谢型谷氨酸受体拮抗Aβ[31-35]诱导的神经元凋亡[J].2008,(20).