Abstract： AIM:To initially investigate the association of elevated insulin stimulated glucose uptake in cardiomycytes during calcium overload.METHODS:111e calcium overload model in cardiomyoctyes was developed by using ionomycin.The glucose uptake of cardiomyoctyes stimulated by insulin was detected with isotopic tracer technique.RESULTS:After calcium overload,cardiomyocyte viability did not decrease significantly(P>0.05,vs control).Cytosolic calcium of cardiomyocytes(ionomycin 1.0 μmol/L)was higher than that of control cardiomyocytes[(376±29)vs(115±21)nmol/L,P<0.05].Insulin stimulated glucose transport into cardiomyocytes.Glucose uptake in 20 U/L insulin group are higher than that in 0.01 U/L insulin group(P<0.05).CONCLUSION:Cardiomyocytes during calcium overload hold responsiveness to insulin,but at the same time,with different concentrations insulin,cardiomyocytes glucose uptake gradually increased during calcium overload.