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Soluble expression and preliminary study on a functional peptide of FnBP protein mediating Staphylococcus aureus invasion
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 18
- DOI:
- No doi available
- Key Word:
- 金黄色葡萄球菌;FnBP蛋白;基因;GST蛋白;基因表达
Abstract： 目的:将介导金黄色葡萄球菌侵袭细胞的关键蛋白FnBP的重要功能肽段与GST蛋白在大肠杆菌中融合表达. 方法:利用合成的特异性引物钓取金黄色葡萄球菌基因组中的目的片段,将其克隆入pGEX-KG载体,在大肠杆菌中与GST蛋白进行融合表达. 结果:成功地表达了目的肽,可溶性的表达产物占细菌可溶性蛋白的20%左右,且目的肽与配基纤连蛋白Fn具有结合的能力. 结论:成功地表达了介导金黄色葡萄球菌侵袭细胞的关键蛋白FnBP中的重要功能肽段,为研究金葡菌侵袭细胞的机制奠定了基础.
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