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Construction and validation of adenovirus expressing dominant negative TGF-β type Ⅰ receptors
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 17
- DOI:
- 10.3321/j.issn:1000-2790.2009.17.004
- Key Word:
- 突变;TGF-β受体;腺病毒;信号转导
Abstract： 建和制备了显性负性突变型TGF-βⅠ型受体腺病毒,其可以感染C3H10细胞并在C3H10细胞中表达相应的显性负性TGF-βⅠ型受体;特定的显性负性突变型TGF-βⅠ型受体腺病毒可分别抑制由BMP2诱导的ALP活性及由TGF-β1诱导的荧光素酶活性.结论:成功构建制备的7种显性负性突变型TGF-βⅠ型受体腺病毒具有抑制相应配体功能的特性,为分析TGF-βⅠ型受体功能及靶向TGF-β信号通路的基因治疗提供了有用的工具.
- [1]王天昱,范宇,李丹,等.TGF-β信号通路在肿瘤发生、发展和转移中的作用[J].2008,(1).
- [2]陈光忠,李铁林,全伟,等.TGFβ1及Ⅰ型受体ALK1/ALK5 mRNA在脑动静脉畸形中的表达及意义[J].2006,(5).
- [3]金天明,武迎红.腺病毒及腺病毒载体的研究进展[J].2007,(2).
- [4]银桂彬,梁杰,罗少军.腺病毒载体的研究进展[J].2007,(5).
- [5]Kamiya N,Mishina Y,Yamauchi M,etc.Disruption of BMP signaling in osteoblasts through type IA receptor (BMPRIA) increases bone mass.[J].2008,23(12).
- [6]Mazerbourg S,Sangkuhl K,Luo CW,etc.Identification of receptors and signaling pathways for orphan bone morphogenetic protein/growth differentiation factor ligands based on genomic analyses[J].2005,280(37).
- [7]Flavell RA,Town T,Laouar Y,etc.Blocking TGF-beta-Smad2/3 innate immune signaling mitigates Alzheimer-like pathology.[J].2008,14(6).
- [8]Guenou H,Marie PJ,Lemonnier J,etc.Bone morphogenetic protein receptor IB signaling mediates apoptosis independently of differentiation in osteoblastic cells.[J].2004,279(3).
- [9]Vittet D,Mallet C,Bailly S,etc.TGFbeta1 induces vasculogenesis and inhibits angiogenic sprouting in an embryonic stem cell differentiation model: respective contribution of ALK1 and ALK5.[J].2006,24(11).