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Anticancer effects and mechanisms of Tanshinon ⅡA sulfonic acid on colon carcinoma cell line HT-29 transplanted subcutaneously in nude mice
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 11
- DOI:
- 10.3321/j.issn:1000-2790.2009.11.004
- Key Word:
- 丹参酮Ⅱ;结肠肿瘤;缺氧诱导因子;血管内皮生长因子;环氧合酶
Abstract： 目的:探讨丹参酮ⅡA(Tan ⅡA)磺酸钠在结肠癌裸鼠移植瘤模型中的抗癌作用及其对肿瘤血管生成的影响.方法:建立结肠癌裸鼠移植瘤模型,随机分成对照组和低、中、高TanⅡA磺酸钠干预组,每组6只.干预组分别用Tan ⅡA磺酸钠1,5,10 mg/kg,ip;对照组等量生理盐水干预.观察抑瘤率,TUNEL法测凋亡指数,CD34标记观察4组瘤块微血管密度(MVD),免疫组化法检测缺氧诱导因子1α(HIF1α)、血管内皮生长因子(VEGF)、环氧合酶2(COX2)的表达.结果:与对照组相比,3组干预组凋亡指数明显上升(均P＜0.05),中剂量组增殖减慢最明显,抑瘤率为(26.0±2.4)%.与对照相比,3组干预组MVD,VEGF表达上调,尤以中高剂量组差异显著(均P＜0.05);3组干预组COX2表达与对照组无统计学差异;HIF1α在低剂量组表达略有下调,但未达到统计学差异,中高剂量组表达则有明显上调(P＜0.05).结论:TanⅡA体内的抗肿瘤效果在中剂量组中最显著,机制口J能与诱导细胞凋亡和上调缺氧诱导因子表达有关.
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