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Reversal effect of oxymatrine and tetrandrine on multidrug resistance in HNE-1(200) cell line
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 11
- DOI:
- 10.3321/j.issn:1000-2790.2009.11.003
- Key Word:
- 氧化苦参碱;粉防己碱;鼻咽肿瘤;HNE-1(200)细胞系;多药耐药
Abstract： 目的:研究天然有效成分氧化苦参碱(Oxy)和粉防己碱(TET)对鼻咽癌耐药细胞株HNE-1(200)的耐药逆转作用.方法:以鼻咽癌耐药细胞株HNE-1(200)为研究对象,用MTT法测定无毒剂量的Oxy和TET作用后HNE-1(200)耐药细胞对常用抗肿瘤药物顺铂(DDP),5-氟尿嘧啶(5-Fu),长春新碱(vincristine,VCR),阿霉素 (Adriamycin,ADM)的增殖抑制率和IC50,并进一步计算其逆转指数.结果:无毒剂量的TET和Oxy分别与4种抗肿瘤药物联合应用后,抗肿瘤药物对耐药细胞的增殖抑制率增加.TET逆转HNE-1(200)细胞对DDP,5-Fu,VCR,ADM的耐药指数分别为2.25,2.1,3.19,2.27;Oxy 逆转HNE-1(200)细胞对DDP,5-Fu,VCR,ADM的耐药指数分别为1.59,1.26,2.28,1.26.结论:低毒浓度的天然药物Oxy和TET对鼻咽耐药细胞株HNE-1(200)具有一定的耐约逆转作用.
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