You Position: Home > Paper
Shear stress induces differentiation of bone marrow mesenchymal stem cells into endothelial cells
( views:38, downloads:0 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 7
- DOI:
- 10.3321/j.issn:1000-2790.2009.07.004
- Key Word:
- 剪应力;骨髓间充质干细胞;内皮细胞;分化
Abstract： 目的:研究剪应力对骨髓间充质干细胞(MSCs)分化的作用.方法:采用平行平板流动腔系统,将人骨髓间充质干细胞在3×10-5,8×10-5,15×10-5N/cm2剪应力水平分别作用24 h,观察细胞形态变化,并进行内皮细胞标记物VonWillebrand factor(vWF)定性间接免疫荧光染色,进行Dil标记的乙酰化LDL(Dil-Ac-LDL)摄取实验以鉴定是否具有内皮细胞功能.结果:在剪应力作用24 h后,MSCs形态变得偏圆、呈多角形,平行于流体方向排列.MSCs在接受3×10-5,8×10-5N/cm2剪应力作用24 h后vWF染色阳性,提示内皮细胞表型特征,同时Dil-Ae-LDL摄取实验阳性,提示具有内皮细胞功能.而接受15×10-5N/cm2剪应力作用的MSCs,vWF染色与Dil-Ac-LDL摄取实验均阴性,提示未出现向内皮细胞分化.结论:剪应力可诱导MSCs向内皮细胞分化,并且与力的大小相关.
- [1]Stock UA,Vacanti JP.Cardiovascular physiology during fetal development and implications for tissue engineering.[J].2001,7(1).
- [2]Sales VL,Sacks MS,Engelmayr GC,etc.Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues[J].2006,27(36).
- [3]Ehninger G,Oswald J,Jorgensen B,etc.Mesenchymal stem cells can be differentiated into endothelial cells in vitro.[J].2004,22(3).
- [4]Kadner A,Hoerstrup SP,Zund G,etc.A new source for cardiovascular tissue engineering: human bone marrow stromal cells.[J].2002,21(6).
- [5]M F, Pittenger,A M, Mackay,S C, Beck,etc.Multilineage potential of adult human mesenchymal stem cells.[J].1999,284(5411).
- [6]Lazarus HM,Koc ON.Mesenchymal stem cells: heading into the clinic.[J].2001,27(3).
- [7]Hao, Wang,Gordon M, Riha,Shaoyu, Yan,etc.Shear stress induces endothelial differentiation from a murine embryonic mesenchymal progenitor cell line.[J].2005,25(9).