Effects of moderate static magnetic field on cell proliferation and cell cycle of leukemia cells
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 5
- DOI:
- 10.3321/j.issn:1000-2790.2009.05.005
- Key Word:
- 静磁场;白血病;细胞增殖;细胞周期
Abstract： 目的:研究0.2-0.4 T中等强度静磁场对人T淋巴细胞白血病细胞及小鼠白血病细胞增殖及细胞周期的影响.方法:人白血病细胞Jurkat clone E6-1和小鼠白血病细胞L1210在0.2～0.4 T中等强度静磁场中培养1,2,3,4,5 d后,分别采用倒置显微镜观察细胞形态变化;Cell Counting Kit-8(CCK-8)法检测细胞增殖能力;流式细胞仪检测细胞周期.结果:曝磁3 d后,与对照组相比,Jurkat clone E6-1细胞团变小,但L1210细胞以单细胞分散生长的特性无明显变化.当细胞接种密度为4×107个/L时,与对照组相比,2种细胞曝磁1,2,3 d后增殖能力均受到明显抑制,细胞增殖抑制率Jurkatclone E6-1细胞分别为14.58%,39.88%,6.77%(P<0.05),L1210细胞分别为8.82%,10.81%,5.40%(P<0.05).然而曝磁4,5 d后,2种细胞的增殖能力明显增强,细胞增殖抑制率Jurkat clone E6-1细胞分别为-8.21%(P<0.05)和-2.49%(P>0.05),L1210细胞分别为-13.01%和-19.46%(P<0.05).当细胞接种密度为8×107个/L时,其结果与4×107个/L接种密度时相反.曝磁1,2,3 d后,2种细胞增殖能力增强,细胞增殖抑制率Jurkat clone K6-1细胞分别为-12.64%,-20.36%,-61.43%(P<0.05),L1210细胞分别为-14.08%,-13.09%,-3.18%(P<0.05).曝磁4,5 d后2种细胞增殖受到抑制,细胞增殖抑制率Jurkat clone E6-1细胞分别为42.20%,95.32%(P<0.05);L1210细胞分别为46.99%,41.97%(P<0.05).曝磁4 d后,与对照组相比,Jurkat cloneE6-1细胞S期所占比例由44.94%上升至77.92%;L1210细胞G2期所占百分比由5.28%上升至9.22%.结论:0.2～0.4 T中等强度静磁场影响了Jurkat clone E6-1,L1210的细胞增殖及细胞周期,且磁场对细胞增殖的抑制作用与细胞密度及曝磁时间有关.
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