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Recombinant E. coli LLO/OVA induces murine BMDC maturation and chemokine and chemokine receptor expression
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 3
- DOI:
- 10.3321/j.issn:1000-2790.2009.03.003
- Key Word:
- 树突细胞;大肠杆茵;趋化因子;趋化因子受体;基因芯片
Abstract： 目的:探讨重组疫苗E. coli LLO/OVA诱导树突状细胞成熟和表达趋化因子及其受体的作用.方法:采用基因芯片杂交及RT-PCR检测经E. coli LLO/OVA刺激后小鼠骨髓树突状细胞(BMDC)的NF-KB信号通路相关分子和趋化因子及其受体mRNA的表达,流式细胞分析BMDC活化状况及趋化因子受体CXCR4的表达.结果:未成熟BMDC表达趋化因子基因Cc12,Cc14,Cc16,Cc19,Cc117,Ccl22,Cxc12,Cxc14和趋化因子受体基因Ccr2,Ccr5和Ccr7.经E. coli LLO/OVA刺激后4-8 h,BMDC明显上调表达基因Myd88,Nf-Icbl,Cc13,Cc15,Cc17,Cc122,Cxc11,Cxc19和Ccr2,同时下调表达趋化因子受体基因Ccr2和Ccr5,但持续表达Ccr7.经该疫苗刺激后8 h,BMDC上调表达基因Tlr2,Myd88,Nf-kbl和Cxcr4.该疫苗刺激后12 h,BMDC下调一系列趋化因子和趋化因子受体基因表达,仅持续表达基因Ccl5和Cxcr4.经疫苗刺激24 h后BMDC上调表达CD40,CD80,CD86,MHC-II类分子及CXCR4.结论:重组疫苗E. coli LLO/OVA可能通过Myd88/NF-KB信号途径诱导了小鼠骨髓树突状细胞成熟并表达一系列趋化因子及趋化因子受体基因,尤其上调了趋化因子受体CXCR4的表达.
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