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Effects of angiopoietin-1 on rhesus macaque choroids-retinal endothelial cells monolayer permeability in high glucose environment
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 3
- DOI:
- 10.3321/j.issn:1000-2790.2009.03.002
- Key Word:
- 脉络膜;视网膜内皮细胞;血管生成素-1;高糖;通透性;猴
Abstract： 目的:利用猴脉络膜一视网膜内皮细胞建立内皮细胞(EC)单层模型,观察高浓度葡萄糖及血管生成素-1(Ang-1)对该模型通透性的影响.方法:待EC于孔径0.8μm的聚碳酸酯微孔滤膜上形成致密单层后分3组进行培养:对照组(DMEM培养基含25 mmol/L葡萄糖)、高糖组(DMEM培养基含30 mmol/L葡萄糖)、Ang-高糖组(DMEM培养基含30mmol/L葡萄糖+0.25 mg/L Ang-1).于1,3,5,7 d 4个时间点,以含白蛋白1 g/L的D-Hanks液灌注滤膜,检测蛋白质渗透压反射系数(8)及滤过系数(K,);并于5d和7d利用琼脂糖凝胶电泳检测DNA ladder.结果:3,5,7 d时,高糖组EC单层的通透性高于对照组(P<0.01);Ang-高糖组与对照组之间Ec单层的通透性无差异(P>.05).高糖组Ec的琼脂糖凝胶电泳在5d及7d均出现DNA ladder,对照组及Ang-高糖组未见DNA ladder.结论:高糖能够促进细胞凋亡,增加Ec单层通透性;Ang-1能抑制EC的凋亡,对抗高糖所致的Ec单层通透性增高.
- [1]J R, Gamble,J, Drew,L, Trezise,etc.Angiopoietin-1 is an antipermeability and anti-inflammatory agent in vitro and targets cell junctions.[J].2000,87(7).
- [2]S M, Baumgartner-Parzer,L, Wagner,M, Pettermann,etc.High-glucose--triggered apoptosis in cultured endothelial cells.[J].1995,44(11).
- [3]Paul M,Funk RH,Hofer A,etc.In situ observation of living pericytes in rat retinal capillaries.[J].1998,56(1).
- [4]G, Thurston,C, Suri,K, Smith,etc.Leakage-resistant blood vessels in mice transgenically overexpressing angiopoietin-1.[J].1999,286(5449).
- [5]A E, Postlethwaite,R, Snyderman,A H, Kang.The chemotactic attraction of human fibroblasts to a lymphocyte-derived factor.[J].1976,144(5).
- [6]Chitra Suri,Pamela F Jones,Sybill Patan,etc.Requisite Role of Angiopoietin-1, a Ligand for the TIE2 Receptor, during Embryonic Angiogenesis[J].1996,87(7).
- [7]R N, Frank.On the pathogenesis of diabetic retinopathy. A 1990 update.[J].1991,98(5).
- [8]Ioffe E,Holash J,Zhou H,etc.Angiopoietin-1 protects the adult vasculature against plasma leakage.[J].2000,6(4).
- [9]T N, Sato,Y, Tozawa,U, Deutsch,etc.Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation.[J].1995,376(6535).
- [10]Altieri DC,Mahboubi K,O'Connor DS,etc.Angiopoietin-1 inhibits endothelial cell apoptosis via the Akt/survivin pathway.[J].2000,275(13).
- [11]Yancopoulos GD,Srikant CB,Guo Y,etc.Mechanisms which mediate the antiapoptotic effects of angiopoietin-1 on endothelial cells.[J].2002,64(1).
- [12]Lorenzi M,Montisano D F,Toledo S,etc.High glucose induces DNA damage in cultured human endothelial cells.[J].1986.
- [13]Graier, W. F.,Grubenthal, I.,Dittrich, P.,etc.Intracellular mechanism of high D-glucose-induced modulation of vascular cell proliferation[J].1995,294(1).
- [14]丁自强.白蛋白对内皮细胞单层屏障功能的增强作用[J].1994,(3).